GST conjugate the tripeptide glutathione to various chemical substrates - often as part of phase II of detoxification. A typical detoxification may involve a P450 adding a hydroxyl group to a substrate (phase I) that then enables a GST to add the glutathione (phase II) which can assist in the excretion of that compound.
The Drosophila enzyme GST D1 showing the insecticide DDT in the active site (white). We confirmed GSTD1 is indeed a DDTase, characterized its crystal structure at high resolution and examined the way the structure changed with addition of DDT using NMR.
Collaborators include Bio21 Institute colleagues: